Animals

2 rabbits were immunized with 1 Antigen (multiple rabbits could be selected)

Adjuvants

Freund's adjuvant

Immune to the original

Provided by the customer, a rabbit requires approximately 3-5mg (concentration ≥1mg/ml) of antigen

Immune method

Immunization with multiple injections at multiple points

Immunization programs

0 days

First immunization

14 days

Secondary immunization

21 days

Third immunization

28 days

Fourth immunization

35 days

Blood was collected and purified

Antibody purification

Delivery of all antisera for purification (antigen affinity purification requires additional antigen from the client), ELISA validation (for immunogens)

A combined

It takes about 50 days (about 2 months) to get purified antibodies

animal

Immunize 1 goat with 1 antigen (choose to immunize multiple goats)

adjuvant

Freund's adjuvant

Immune to the original

As provided by the customer, a sheep requires approximately 5-8mg (concentration ≥1mg/ml) of antigen

Immune method

Immunization with multiple injections at multiple points

Immunization programs

0 days

The first immunization

21 days

The second immunization

35 days

The third immunization

49 days

The fourth immunization  

63 days

The fifth immunization 

70 days

Serum sample collection (simultaneous detection by both parties)

72 days

Whether to conduct the sixth immunization or extract all serum will be decided according to the results of both parties

Antibody purification

After the rabbit serum titer reached the standard (ELISA titer reached 1:80,000 or more), blood was collected and all antiserum was delivered for purification of antibodies (antigen affinity purification requires the customer to provide additional antigens) and ELISA verification (for immunogens).

A combined

It takes about 80 days (two and a half months) to get purified antibodies