Reactivity

Species independent

Applications

WB; IF/ICC

Alternative Names

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.07% sodium azide.

Source

Mouse

Dilution

WB: 1:500-1:2000; IF:1:50-1:200

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1mg/ml

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles.

Subcellular Location

-

MW

N/A

Background

Epitope tagging is a widely accepted technique that involves the fusion of an epitope labeled peptide to a certain protein as a marker for gene expression. This technique allows gene expression to be easily monitored during western blotting, immunoprecipitation and immunofluorescence by using an antibody that recognizes such an epitope. Amino acid sequences that are widely used for epitope tagging are as follows; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product, and Vesicular stomatitis virus glycoprotein, respectively.

Swiss-Prot

N/A