Reactivity

Species independent

Applications

WB

Alternative Names

ECFPTag; ECFPTag; tagECFP; Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein; ECFP-Tag.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.07% sodium azide.

Source

Mouse

Dilution

WB: 1:2000-1:5000

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

N/A

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles.

Subcellular Location

-

MW

N/A

Background

Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively.

Swiss-Prot 

N/A