Reactivity

Species independent

Applications

WB; IP; IF/ICC

Alternative Names

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.07% sodium azide.

Source

Mouse

Dilution

WB: 1:3000; IP: 1:200; IF: 1:1000

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

N/A

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles.

Subcellular Location

-

MW

N/A

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Swiss-Prot 

IDS0065