Reactivity

Mouse; Rat

Applications

WB; IHC-p; IF(paraffin section)

Alternative Names

Alpha-actinin-1; Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1; ACTN1

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

WB: 1:1000-2000; IHC: 1:50-1:200; IF: 1:50-1:200

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

N/A

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles.

Subcellular Location

-

MW

~ 103 kDa

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Swiss-Prot           

P12814