Reactivity

Human; Mouse; Rat

Applications

WB; IHC; IF; ELISA

Alternative Names

CCAAT/enhancer-binding protein alpha

Immunogen

The antiserum was produced against synthesized peptide derived from human C/EBP-alpha. AA range:6-55

Source

Rabbit

Dilution

WB: 1/500 - 1/2000; IHC: 1/100 - 1/300; ELISA: 1/5000; IF 1:50-200

Purification

The antibody was affinity-purified from rabbit antiserum by

affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.

Subcellular Location

Nucleus .; [Isoform 4]: Nucleus, nucleolus .

MW

42 kDa

Background

· This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.

Swiss-Prot

P49715