Reactivity

Human; Mouse

Applications

WB; IHC-p

Alternative Names

Methylcytosine dioxygenase TET2 (EC 1.14.11.n2)

Immunogen

Synthesized peptide derived from human C-ternal TET2

Source

Rabbit

Dilution

WB 1:500-2000 IHC 1:50-200

Purification

The antibody was affinity-purified from rabbit serum by

affinity-chromatography using specific immunogen.

Concentration

1 mg/mL

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.

Subcellular Location

Nucleus

MW

220 kDa

Background

· Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

Swiss-Prot

Q6N021