Reactivity

Human; Mouse; Rat

Applications

WB; IHC-p; IF(paraffin section); ELISA

Alternative Names

Transcription factor p65

Immunogen

The antiser,um was produced against synthesized peptide derived from human NF-kappaB p65. AA range:247-296

Source

Rabbit

Dilution

WB: 1:500 - 1:2000; IHC: 1:100 - 1:300; ELISA: 1/5000; Not yet tested in other applications.

Purification

The  antibody  was  affinity-purified  from  rabbit  antiserum  by  affinity- chromatography using epitope-specific immunogen

Concentration

1 mg/ml

Storage&Stability

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.

Subcellular Location

Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection.

MW

60 kDa

Background

RELA proto-oncogene, NF-kB subunit(RELA)        Homo sapiens    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Swiss-Prot

Q04206